Using five transplantable murine tumors (SCC-VII, B16F1, KHT-C, KHT-LP1, RIF-1), measurements of tumor hypoxia have been made with two techniques which have the potential to be used for assessing oxygenation in human tumors (the Eppendorf$p{\rm O}_{2}$ Histograph and binding of$[{}^{3}{\rm H}]\text{misonidazole}$) and have been compared with an established radiobiological technique, the paired survival assay. There were significant differences in the$p{\rm O}_{2}$ measurements made in individual tumors both within and between the five different tumor types. Significant differences between the tumor types were also found for the$[{}^{3}{\rm H}]\text{misonidazole}$ binding. A correlation was observed between the mean values of the hypoxic proportion as measured by the paired survival assay and the mean binding of$[{}^{3}{\rm H}]\text{misonidazole}$ as measured by both tumor activity in dpm/100 mg tissue (r = 0.94, P = 0.02) and the tumor-to-muscle activity ratio (r = 0.87, P = 0.06). No biologically significant correlation was seen between the mean values of the hypoxic proportion from anesthetized mice as measured by the paired survival assay (range 20-58%) and the pooled Eppendorf$p{\rm O}_{2}$ Histograph measurements made on groups of tumors. These results with the Eppendorf$p{\rm O}_{2}$ Histograph are similar to those reported by others. When both Eppendorf$p{\rm O}_{2}$ Histograph measurements and paired survival measurements were made on the same individual KHT-C tumors, it was again found that there was no correlation between the two measurements of hypoxia.
Skip Nav Destination
Close
Article navigation
April 1996
Research Article|
April 01 1996
Comparing Techniques of Measuring Tumor Hypoxia in Different Murine Tumors: Eppendorf$p{\rm O}_{2}$ Histograph,$[{}^{3}{\rm H}]\text{Misonidazole}$ Binding and Paired Survival Assay
Radiat Res (1996) 145 (4): 491–500.
Citation
Mary-Claire Kavanagh, Alex Sun, Qiyue Hu, Richard P. Hill; Comparing Techniques of Measuring Tumor Hypoxia in Different Murine Tumors: Eppendorf$p{\rm O}_{2}$ Histograph,$[{}^{3}{\rm H}]\text{Misonidazole}$ Binding and Paired Survival Assay. Radiat Res 1 April 1996; 145 (4): 491–500. doi: https://doi.org/10.2307/3579071
Download citation file:
Close
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Sign in via your Institution
Sign in via your InstitutionCiting articles via
Commonalities Between COVID-19 and Radiation Injury
Carmen I. Rios, David R. Cassatt, Brynn A. Hollingsworth, Merriline M. Satyamitra, Yeabsera S. Tadesse, Lanyn P. Taliaferro, Thomas A. Winters, Andrea L. DiCarlo
Low-Dose Radiation Therapy (LDRT) for COVID-19: Benefits or Risks?
Pataje G. Prasanna, Gayle E. Woloschak, Andrea L. DiCarlo, Jeffrey C. Buchsbaum, Dörthe Schaue, Arnab Chakravarti, Francis A. Cucinotta, Silvia C. Formenti, Chandan Guha, Dale J. Hu, Mohammad K. Khan, David G. Kirsch, Sunil Krishnan, Wolfgang W. Leitner, Brian Marples, William McBride, Minesh P. Mehta, Shahin Rafii, Elad Sharon, Julie M. Sullivan, Ralph R. Weichselbaum, Mansoor M. Ahmed, Bhadrasain Vikram, C. Norman Coleman, Kathryn D. Held
Germicidal Efficacy and Mammalian Skin Safety of 222-nm UV Light
Manuela Buonanno, Brian Ponnaiya, David Welch, Milda Stanislauskas, Gerhard Randers-Pehrson, Lubomir Smilenov, Franklin D. Lowy, David M. Owens, David J. Brenner