Using five transplantable murine tumors (SCC-VII, B16F1, KHT-C, KHT-LP1, RIF-1), measurements of tumor hypoxia have been made with two techniques which have the potential to be used for assessing oxygenation in human tumors (the Eppendorf$p{\rm O}_{2}$ Histograph and binding of$[{}^{3}{\rm H}]\text{misonidazole}$) and have been compared with an established radiobiological technique, the paired survival assay. There were significant differences in the$p{\rm O}_{2}$ measurements made in individual tumors both within and between the five different tumor types. Significant differences between the tumor types were also found for the$[{}^{3}{\rm H}]\text{misonidazole}$ binding. A correlation was observed between the mean values of the hypoxic proportion as measured by the paired survival assay and the mean binding of$[{}^{3}{\rm H}]\text{misonidazole}$ as measured by both tumor activity in dpm/100 mg tissue (r = 0.94, P = 0.02) and the tumor-to-muscle activity ratio (r = 0.87, P = 0.06). No biologically significant correlation was seen between the mean values of the hypoxic proportion from anesthetized mice as measured by the paired survival assay (range 20-58%) and the pooled Eppendorf$p{\rm O}_{2}$ Histograph measurements made on groups of tumors. These results with the Eppendorf$p{\rm O}_{2}$ Histograph are similar to those reported by others. When both Eppendorf$p{\rm O}_{2}$ Histograph measurements and paired survival measurements were made on the same individual KHT-C tumors, it was again found that there was no correlation between the two measurements of hypoxia.

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