The molecular mechanisms underlying radiation-induced defects in the bone marrow which may contribute to the development of radiation-induced hematopoietic disorders such as aplastic anemia and leukemia are not known. Persistent changes in gene expression were examined after exposure of cells of a murine bone marrow stromal cell line to ionizing radiation. Analysis of mRNA transcript levels by differential display led to the identification of a band, C122, which increased in abundance 1 week after exposure. Northern blot hybridization verified these results and revealed a 12-fold increase in abundance of this message for up to 3 weeks after irradiation in vitro. DNA sequence analysis identified clone C122 as murine serum amyloid A 3 (Saa3), a member of the Saa family of acute-phase or inflammatory response genes. Saa message levels were then examined in vivo in the bone marrow of mice exposed to total-body irradiation. Semi-quantitative reverse transcription-polymerase chain reaction revealed a 15-20-fold increase in Saa3 message levels in the bone marrow of irradiated mice from 3 days to 2 weeks after exposure. Saa3 message levels continued to be 2-3-fold above control for up to 28 weeks in vivo. Two additional members of the murine Saa gene family, Saa1 and Saa2, were also detected in irradiated bone marrow. The expression of SAA1 and SAA2 was also detected in irradiated cells of human bone marrow stromal cell lines in vitro. These results suggest that SAA genes are involved in the radiation response in the bone marrow, but their role in the recovery of the marrow after irradiation or in the development of radiation-induced hematopoietic disorders remains to be established. Additionally, the persistent radiation-induced increase in expression suggests the potential utility of using SAA3 transcript levels as a molecular marker of past radiation exposure.
Skip Nav Destination
Close
Article navigation
June 1998
Research Article|
June 01 1998
Induction of Serum Amyloid A Inflammatory Response Genes in Irradiated Bone Marrow Cells
Radiat Res (1998) 149 (6): 570–578.
Citation
Kristin L. Goltry, Michael W. Epperly, Joel S. Greenberger; Induction of Serum Amyloid A Inflammatory Response Genes in Irradiated Bone Marrow Cells. Radiat Res 1 June 1998; 149 (6): 570–578. doi: https://doi.org/10.2307/3579903
Download citation file:
Close
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Sign in via your Institution
Sign in via your InstitutionCiting articles via
Commonalities Between COVID-19 and Radiation Injury
Carmen I. Rios, David R. Cassatt, Brynn A. Hollingsworth, Merriline M. Satyamitra, Yeabsera S. Tadesse, Lanyn P. Taliaferro, Thomas A. Winters, Andrea L. DiCarlo
Low-Dose Radiation Therapy (LDRT) for COVID-19: Benefits or Risks?
Pataje G. Prasanna, Gayle E. Woloschak, Andrea L. DiCarlo, Jeffrey C. Buchsbaum, Dörthe Schaue, Arnab Chakravarti, Francis A. Cucinotta, Silvia C. Formenti, Chandan Guha, Dale J. Hu, Mohammad K. Khan, David G. Kirsch, Sunil Krishnan, Wolfgang W. Leitner, Brian Marples, William McBride, Minesh P. Mehta, Shahin Rafii, Elad Sharon, Julie M. Sullivan, Ralph R. Weichselbaum, Mansoor M. Ahmed, Bhadrasain Vikram, C. Norman Coleman, Kathryn D. Held
Germicidal Efficacy and Mammalian Skin Safety of 222-nm UV Light
Manuela Buonanno, Brian Ponnaiya, David Welch, Milda Stanislauskas, Gerhard Randers-Pehrson, Lubomir Smilenov, Franklin D. Lowy, David M. Owens, David J. Brenner
Photon GRID Radiation Therapy: A Physics and Dosimetry White Paper from the Radiosurgery Society (RSS) GRID/LATTICE, Microbeam and FLASH Radiotherapy Working Group
Hualin Zhang, Xiaodong Wu, Xin Zhang, Sha X. Chang, Ali Megooni, Eric D. Donnelly, Mansoor M. Ahmed, Robert J. Griffin, James S. Welsh, Charles B. Simone, II, Nina A. Mayr