Chromosome breakpoints induced by neutrons or γ rays in Chinese hamster ovary cells were mapped to Giemsa-light or Giemsa-dark bands or to band junctions. Radiation-induced breakpoints were found to be distributed nonrandomly according to chromosome or band length. More than 60% of the breakpoints were localized in G-light bands. A group of 13 bands which corresponded to only 7% of the total chromosome length contained 22% of the breakpoints produced by neutrons and 14% of those induced by γ rays. Seven of these 13 bands are also preferentially damaged by AluI, BamHI and DNase I as reported previously. The results indicate that chromatin and nuclear structure may play a role in the distribution of breakpoints produced by ionizing radiation and endonucleases.
Intrachromosomal Localization of Aberration Breakpoints Induced by Neutrons and Gamma Rays in Chinese Hamster Ovary Cells
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Wilner Martínez-López, Enrique M. Boccardo, Gustavo A. Folle, Valentina Porro, Günter Obe; Intrachromosomal Localization of Aberration Breakpoints Induced by Neutrons and Gamma Rays in Chinese Hamster Ovary Cells. Radiat Res 1 November 1998; 150 (5): 585–592. doi: https://doi.org/10.2307/3579876
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