M10 cells, which are deficient in the repair of DNA DSBs and are therefore radiosensitive, are about twofold more thermoresistant than their parental L5178Y cells. We found that, after heat shock at 43°C under conditions resulting in 10% survival (<tex-math>$D_{10}$</tex-math>), M10 cells did not undergo apoptosis, whereas L5178Y cells did undergo apoptosis. M10 cells, but not L5178Y cells, constitutively expressed Hsp72 protein. Moreover, the M10 cells accumulated higher amounts of the heat-inducible form of Hsp72. The patterns of activation of the DNA-binding activity of HSF (heat-shock factor) differed in M10 and L5178Y cells. In response to heat shock, M10 cells accumulated greater amounts of Trp53 protein (formerly known as p53) than the parental cells. Cdkn1a (formerly known as p21, Waf1) was constitutively expressed and further accumulated after heat shock only in M10 cells. We suggest that heat-inducible Hsp72 to a larger extent, and constitutive Hsp72 to a lesser extent, together with Cdkn1a may be involved in the protection of M10 cells against heat-induced apoptosis. Apoptosis in these cells is likely to occur in Trp53-dependent manner.
Skip Nav Destination
Article navigation
September 1999
Research Article|
September 01 1999
Increased Resistance of the Radiosensitive M10 Mutant Cells of the L5178Y Mouse Lymphoma Cell Line to Heat-Induced Apoptosis
Radiat Res (1999) 152 (3): 321–327.
Citation
Valentina V. Ostapenko, Xinjiang Wang, Ken Ohnishi, Akihisa Takahashi, Itsuo Yamamoto, Yoshimasa Tanaka, Takeo Ohnishi; Increased Resistance of the Radiosensitive M10 Mutant Cells of the L5178Y Mouse Lymphoma Cell Line to Heat-Induced Apoptosis. Radiat Res 1 September 1999; 152 (3): 321–327. doi: https://doi.org/10.2307/3580332
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionCiting articles via
Commonalities Between COVID-19 and Radiation Injury
Carmen I. Rios, David R. Cassatt, Brynn A. Hollingsworth, Merriline M. Satyamitra, Yeabsera S. Tadesse, Lanyn P. Taliaferro, Thomas A. Winters, Andrea L. DiCarlo
Studies of the Mortality of Atomic Bomb Survivors, Report 14, 1950–2003: An Overview of Cancer and Noncancer Diseases
Kotaro Ozasa, Yukiko Shimizu, Akihiko Suyama, Fumiyoshi Kasagi, Midori Soda, Eric J. Grant, Ritsu Sakata, Hiromi Sugiyama, Kazunori Kodama
Effects of Radiation on Blood Pressure and Body Weight in the Spontaneously Hypertensive Rat Model. Are Radiation Effects on Blood Pressure Affected by Genetic Background?
Norio Takahashi, Munechika Misumi, Yasuharu Niwa, Hideko Murakami, Waka Ohishi, Toshiya Inaba, Akiko Nagamachi, Satoshi Tanaka, Ignacia Braga Tanaka, III, Gen Suzuki
Long-Term Effects of the Rain Exposure Shortly after the Atomic Bombings in Hiroshima and Nagasaki
Ritsu Sakata, Eric J. Grant, Kyoji Furukawa, Munechika Misumi, Harry Cullings, Kotaro Ozasa, Roy E. Shore
Exposure to Ionizing Radiation and Risk of Dementia: A Systematic Review and Meta-Analysis
Tanvi Srivastava, Ekaterina Chirikova, Sapriya Birk, Fanxiu Xiong, Tarek Benzouak, Jane Y. Liu, Paul J. Villeneuve, Lydia B. Zablotska