Hematopoietic processes, especially megakaryocytopoiesis and thrombopoiesis, are highly sensitive to extracellular oxidative stresses such as ionizing radiation and chemotherapeutic agents. This study examined the terminal maturation of megakaryocytes and platelet production in hematopoietic stem/progenitor cells (HSPCs) exposed to ionizing radiation. Highly purified CD34+ cells derived from human placental/umbilical cord blood were exposed to X rays (2 Gy, 150 kVp, 20 mA; 0.5-mm aluminum and 0.3-mm copper filters) at a dose rate of approximately 1 Gy/min and then cultured in a serum-free medium supplemented with thrombopoietin and interleukin-3. The number of cells generated from X-irradiated CD34+ cells decreased with the time in culture. However, the fraction of CD34+Tie-2+ and CD41+Tie-2+ cells among the total cells generated from X-irradiated cells increased significantly in comparison to nonirradiated controls on day 7. In addition, the CD42a+ particles, which appeared to be platelets, generated from the X-irradiated HSPCs appeared to be normal. Quantitative real-time reverse transcriptase-polymerase chain reaction analysis of the expression of various genes in cells harvested from the cultures showed that the early hematopoiesis-related genes FLI1, HOXB4 and Tie-2, the cytokine receptor genes KIT and IL3RA, and the oxidative stress-related genes HO1 and NQO1 were upregulated on day 7. These results suggest that normal terminal maturation of megakaryocytes and platelet production occur in residual HSPCs after exposure to ionizing radiation despite the adverse effect of radiation on proliferation and differentiation of HSPCs. Ionizing radiation may have the potential to promote both megakaryocytopoiesis and thrombopoiesis.
Skip Nav Destination
Article navigation
1 December 2011
REGULAR ARTICLES|
October 25 2011
Megakaryocytopoiesis and Thrombopoiesis in Hematopoietic Stem Cells Exposed to Ionizing Radiation Available to Purchase
Satoru Monzen;
Satoru Monzen
Department of Radiological Life Sciences, Hirosaki University Graduate School of Health Sciences, 66-1 Hon-cho, Hirosaki, Aomori 036-8564, Japan
Search for other works by this author on:
Emiko Tashiro;
Emiko Tashiro
Department of Radiological Life Sciences, Hirosaki University Graduate School of Health Sciences, 66-1 Hon-cho, Hirosaki, Aomori 036-8564, Japan
Search for other works by this author on:
Ikuo Kashiwakura
Ikuo Kashiwakura
1
Department of Radiological Life Sciences, Hirosaki University Graduate School of Health Sciences, 66-1 Hon-cho, Hirosaki, Aomori 036-8564, Japan
1Address for correspondence: Department of Radiological Life Sciences, Hirosaki University Graduate School of Health Sciences, 66-1 Hon-cho, Hirosaki 036-8564, Japan; e-mail: [email protected].
Search for other works by this author on:
Radiat Res (2011) 176 (6): 716–724.
Article history
Received:
July 06 2011
Accepted:
September 27 2011
Citation
Satoru Monzen, Emiko Tashiro, Ikuo Kashiwakura; Megakaryocytopoiesis and Thrombopoiesis in Hematopoietic Stem Cells Exposed to Ionizing Radiation. Radiat Res 1 December 2011; 176 (6): 716–724. doi: https://doi.org/10.1667/RR2725.1
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionCiting articles via
Dosimetry: Was and Is an Absolute Requirement for Quality Radiation Research
Daniel Johnson, H. Harold Li, Bruce F. Kimler
Hepatic Stellate Cell-mediated Increase in CCL5 Chemokine Expression after X-ray Irradiation Determined In Vitro and In Vivo
Masataka Taga, Kengo Yoshida, Shiho Yano, Keiko Takahashi, Seishi Kyoizumi, Megumi Sasatani, Keiji Suzuki, Tomohiro Ogawa, Yoichiro Kusunoki, Tatsuaki Tsuruyama
Lethality Assessments for Acutely Irradiated Cynomolgus Macaques Under Subject-based Care
Issa Melendez-Miranda, Oluseyi O. Fatanmi, Stephen Y. Wise, Sarah A. Petrus, Alana D. Carpenter, Cara Olsen, Artur A. Serebrenik, Luis A. Lugo-Roman, Thomas M. Seed, Michael D. Kaytor, Vijay K. Singh
Germicidal Efficacy and Mammalian Skin Safety of 222-nm UV Light
Manuela Buonanno, Brian Ponnaiya, David Welch, Milda Stanislauskas, Gerhard Randers-Pehrson, Lubomir Smilenov, Franklin D. Lowy, David M. Owens, David J. Brenner
Radiofrequency Fields and Calcium Movements Into and Out of Cells
Andrew Wood, Ken Karipidis