It has been suggested that a mechanistic understanding of the cellular responses to low dose and dose rate may be valuable in reducing some of the uncertainties involved in current risk estimates for cancer- and non-cancer-related radiation effects that are inherited in the linear no-threshold hypothesis. In this study, the effects of low-dose radiation on the proteome in both human fibroblasts and stem cells were investigated. Particular emphasis was placed on examining: 1. the dose-response relationships for the differential expression of proteins in the low-dose range (40–140 mGy) of low-linear energy transfer (LET) radiation; and 2. the effect on differential expression of proteins of a priming dose given prior to a challenge dose (adaptive response effects). These studies were performed on cultured human fibroblasts (VH10) and human adipose-derived stem cells (ADSC). The results from the VH10 cell experiments demonstrated that low-doses of low-LET radiation induced unique patterns of differentially expressed proteins for each dose investigated. In addition, a low priming radiation dose significantly changed the protein expression induced by the subsequent challenge exposure. In the ADSC the number of differentially expressed proteins was markedly less compared to VH10 cells, indicating that ADSC differ in their intrinsic response to low doses of radiation. The proteomic results are further discussed in terms of possible pathways influenced by low-dose irradiation.
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1 March 2016
Research Article|
March 02 2016
Differential Response and Priming Dose Effect on the Proteome of Human Fibroblast and Stem Cells Induced by Exposure to Low Doses of Ionizing Radiation
Monika Hauptmann
;
Monika Hauptmann
1
aFederal Office for Radiation Protection, Department SG Radiation Protection and Health, Oberschleissheim, Germany;
1Address for correspondence: Federal Office for Radiation Protection, Department SG Radiation Protection and Health, Ingolstädter Landstraße 1, 85764 Oberschleissheim, Germany; email: mhauptmann@bfs.de.
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Siamak Haghdoost
;
Siamak Haghdoost
cCenter for Radiation Protection Research, Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Stockholm, Sweden;
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Maria Gomolka
;
Maria Gomolka
aFederal Office for Radiation Protection, Department SG Radiation Protection and Health, Oberschleissheim, Germany;
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Hakan Sarioglu
;
Hakan Sarioglu
bHelmholtz Zentrum München, German Research Center for Environmental Health, Department of Protein Science, Neuherberg, Germany;
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Marius Ueffing
;
Marius Ueffing
bHelmholtz Zentrum München, German Research Center for Environmental Health, Department of Protein Science, Neuherberg, Germany;
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Anne Dietz
;
Anne Dietz
aFederal Office for Radiation Protection, Department SG Radiation Protection and Health, Oberschleissheim, Germany;
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Ulrike Kulka
;
Ulrike Kulka
aFederal Office for Radiation Protection, Department SG Radiation Protection and Health, Oberschleissheim, Germany;
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Kristian Unger
;
Kristian Unger
dHelmholtz Zentrum München, German Research Center for Environmental Health, Department of Radiation Cytogenetics, Neuherberg, Germany; and
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Gabriele Babini
;
Gabriele Babini
eDepartment of Physics, University of Pavia, Pavia, Italy
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Mats Harms-Ringdahl
;
Mats Harms-Ringdahl
cCenter for Radiation Protection Research, Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Stockholm, Sweden;
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Andrea Ottolenghi
;
Andrea Ottolenghi
eDepartment of Physics, University of Pavia, Pavia, Italy
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Sabine Hornhardt
Sabine Hornhardt
aFederal Office for Radiation Protection, Department SG Radiation Protection and Health, Oberschleissheim, Germany;
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Radiat Res (2016) 185 (3): 299–312.
Article history
Received:
August 11 2015
Accepted:
January 14 2016
Citation
Monika Hauptmann, Siamak Haghdoost, Maria Gomolka, Hakan Sarioglu, Marius Ueffing, Anne Dietz, Ulrike Kulka, Kristian Unger, Gabriele Babini, Mats Harms-Ringdahl, Andrea Ottolenghi, Sabine Hornhardt; Differential Response and Priming Dose Effect on the Proteome of Human Fibroblast and Stem Cells Induced by Exposure to Low Doses of Ionizing Radiation. Radiat Res 1 March 2016; 185 (3): 299–312. doi: https://doi.org/10.1667/RR14226.1
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