The goal of this study was to determine whether in vivo X irradiation induces nontargeted effects, such as delayed effects and bystander effects in ICR mouse lymphocytes. We first examined the generation of DNA double-strand breaks (DSBs) in lymphocytes, isolated from ICR mice exposed to 1 Gy X irradiation, by enumeration of p53 binding protein 1 (53BP1) foci, and observed that the number of 53BP1 foci reached their maximum 3 days postirradiation and decreased to background level 30 days postirradiation. However, the number of 53BP1 foci was significantly increased in lymphocytes isolated from ICR mice 90–365 days postirradiation. This result indicates that in vivo X irradiation induced delayed DSBs in ICR mouse lymphocytes. We next counted the number of 53BP1 foci in lymphocytes isolated from sham-irradiated ICR mice that had been co-cultured with lymphocytes isolated from 1 Gy X-irradiated ICR mice, and observed a significant increase in the number of 53BP1 foci 1–7 days postirradiation. This result indicates that in vivo X irradiation induced bystander effects in ICR mouse lymphocytes. These findings suggest that in vivo X irradiation induces early and delayed nontargeted effects in ICR mouse lymphocytes.
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1 July 2016
Research Article|
June 22 2016
Early and Delayed Induction of DSBs by Nontargeted Effects in ICR Mouse Lymphocytes after In Vivo X Irradiation
Mitsuaki Ojima
;
Mitsuaki Ojima
1
aDepartment of Environmental Health Science, Oita University of Nursing and Health Sciences, Oita 840-1201, Japan
1Address for correspondence: Department of Environmental Health Science, Oita University of Nursing and Health Sciences, 2944-9 Megusuno, Oita 840-1201, Japan; email: ojima@oita-nhs.ac.jp.
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Keiko Iwashita
;
Keiko Iwashita
aDepartment of Environmental Health Science, Oita University of Nursing and Health Sciences, Oita 840-1201, Japan
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Genro Kashino
;
Genro Kashino
bAdvanced Molecular Center, Faculty of Medicine, Oita University, Yufu 879-5593, Japan
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Shinko Kobashigawa
;
Shinko Kobashigawa
bAdvanced Molecular Center, Faculty of Medicine, Oita University, Yufu 879-5593, Japan
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Noriko Sasano
;
Noriko Sasano
aDepartment of Environmental Health Science, Oita University of Nursing and Health Sciences, Oita 840-1201, Japan
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Akiko Takeshita
;
Akiko Takeshita
aDepartment of Environmental Health Science, Oita University of Nursing and Health Sciences, Oita 840-1201, Japan
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Nobuhiko Ban
;
Nobuhiko Ban
cTokyo Healthcare University, Tokyo 152-8558, Japan
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Michiaki Kai
Michiaki Kai
aDepartment of Environmental Health Science, Oita University of Nursing and Health Sciences, Oita 840-1201, Japan
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Radiat Res (2016) 186 (1): 65–70.
Article history
Received:
February 18 2015
Accepted:
May 24 2016
Citation
Mitsuaki Ojima, Keiko Iwashita, Genro Kashino, Shinko Kobashigawa, Noriko Sasano, Akiko Takeshita, Nobuhiko Ban, Michiaki Kai; Early and Delayed Induction of DSBs by Nontargeted Effects in ICR Mouse Lymphocytes after In Vivo X Irradiation. Radiat Res 1 July 2016; 186 (1): 65–70. doi: https://doi.org/10.1667/RR14053.1
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