The production of 2-deoxyribonolactones (C1′-oxidation product), C4′-oxidized abasic sites and C5′-carbonyl terminated strand scission products was investigated in complexes of double-stranded DNA with protamine, poly-L-lysine and spermine exposed to X-ray radiation. The lesions were quantified by high-performance liquid chromatography through the release of the corresponding low-molecular-weight products 5-methylenefuran-2(5H)-one, N-(2′-hydroxy-ethyl)-5-methylene-D3-pyrrolin-2-one and furfural, respectively. All binders were found to increase the relative yield of C1′ oxidation up to 40% of the total 2-deoxyribose damage through the indirect effect versus approximately 18% typically found in homogeneous solutions by the same technique. On the contrary, the yield of C5′-oxidation was found to be suppressed almost completely, while in homogeneous solutions it constituted approximately 14% of the total. The observed change in end product distribution is attributed to free valence transfer to and from the complexing agent, although the mechanisms associated with this process remain unclear.
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1 September 2019
Research Article|
July 12 2019
Association with Polyamines and Polypeptides Increases the Relative Yield of 2-Deoxyribonolactone Lesions in Radiation-Damaged DNA
Yuriy Razskazovskiy
;
Yuriy Razskazovskiy
1
Departments of aPhysics and Astronomy
1 Address for correspondence: Department of Physics and Astronomy, Box 70652, East Tennessee State University, Johnson City, TN 37614; email: raiskazy@etsu.edu.
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Modeste Tegomoh
;
Modeste Tegomoh
2
bDepartments of Chemistry, East Tennessee State University, Johnson City, Tennessee 37614
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Marina Roginskaya
Marina Roginskaya
bDepartments of Chemistry, East Tennessee State University, Johnson City, Tennessee 37614
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Radiat Res (2019) 192 (3): 324–330.
Article history
Received:
April 01 2019
Accepted:
June 06 2019
Citation
Yuriy Razskazovskiy, Modeste Tegomoh, Marina Roginskaya; Association with Polyamines and Polypeptides Increases the Relative Yield of 2-Deoxyribonolactone Lesions in Radiation-Damaged DNA. Radiat Res 1 September 2019; 192 (3): 324–330. doi: https://doi.org/10.1667/RR15396.1
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