Photodynamic therapy (PDT) uses a combination of photosensitizers with visible light to generate reactive species and selectively kill tumor or unwanted tissue. Znln2S4 nanoparticles are widely implemented in photovoltaic device materials and photolysis water catalysts owing to their unique photoelectric properties. Whether Znln2S4 itself can be used as an effective dye in PDT is still unknown. To determine the effects and potential mechanism of Znln2S4PDT on HepG2 cell apoptosis, electron microscopic analysis was performed to monitor the apoptotic morphology of HepG2 cells upon exposure to Znln2S4-PDT. Flow cytometry was performed to measure the apoptosis rate and intracellular ROS production. Western blot and ELISA were performed to reveal the expression changes in Bax, caspase-3 and caspase-9. Data from this work suggested that cells exhibited the typical apoptotic morphology in response to Znln2S4-PDT, with high apoptotic rate. The intracellular ROS production after Znln2S4-PDT occurred in a dose-dependent manner. Moreover, Znln2S4-PDT augmented the expression levels of pro-apoptosis factors, especially, Bax, caspase-3 and caspase-9. Taken together, our novel findings, Znln2S4-PDT elicited HepG2 cell apoptosis, suggesting Znln2S4 as a promising photosensitizer candidate for cancer therapy.
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1 October 2019
Research Article|
October 01 2019
A Novel Photosensitizer Znln2S4 Mediated Photodynamic Therapy Induced-HepG2 Cell Apoptosis
Yongfa Li;
Yongfa Li
aThe Second School of Clinical Medicine, Yangtze University, Jingzhou, Hubei, China
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Yanhua Xu;
Yanhua Xu
bDepartment of Oncology, Jingzhou Central Hospital, Jingzhou, Hubei, China
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Xiaochun Peng;
Xiaochun Peng
aThe Second School of Clinical Medicine, Yangtze University, Jingzhou, Hubei, China
cDepartment of Biochemistry and Molecular Biology, The Second School of Clinical Medicine, Health Science Center, Yangtze University, Jingzhou, Hubei 434023, China
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Jangrong Huang;
Jangrong Huang
aThe Second School of Clinical Medicine, Yangtze University, Jingzhou, Hubei, China
cDepartment of Biochemistry and Molecular Biology, The Second School of Clinical Medicine, Health Science Center, Yangtze University, Jingzhou, Hubei 434023, China
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Minquan Yang;
Minquan Yang
dDepartment of Electrical and Computer Engineering, National University of Singapore, 117583, Singapore, Singapore
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Xianwang Wang
Xianwang Wang
1
aThe Second School of Clinical Medicine, Yangtze University, Jingzhou, Hubei, China
cDepartment of Biochemistry and Molecular Biology, The Second School of Clinical Medicine, Health Science Center, Yangtze University, Jingzhou, Hubei 434023, China
1Address for correspondence: Yangtze University, The Second School of Clinical Medicine, 1 Nanhuan Road, Jingzhou, Hubei 434023, China; email: huangjr999@sina.com (JH), eleymin@nus.edu.sg (MY) or 500851@yangtzeu.edu.cn (XW).
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Radiat Res (2019) 192 (4): 422–430.
Article history
Received:
March 28 2019
Accepted:
June 25 2019
Citation
Yongfa Li, Yanhua Xu, Xiaochun Peng, Jangrong Huang, Minquan Yang, Xianwang Wang; A Novel Photosensitizer Znln2S4 Mediated Photodynamic Therapy Induced-HepG2 Cell Apoptosis. Radiat Res 1 October 2019; 192 (4): 422–430. doi: https://doi.org/10.1667/RR15389.1
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