Radiotherapy to treat brain tumors can potentially harm the central nervous system (CNS). The radiation stimulates a series of immune responses in both the CNS as well as peripheral immune system. To date, studies have mostly focused on the changes occurring in the immune response within the CNS. In this study, we investigated the effect of γ-ray-induced CNS injury on the peripheral immune response using a cell co-culture model and a whole-brain irradiation (WBI) rat model. Nerve cells (SH-SY5Y and U87 MG cells) were γ-ray irradiated, then culture media of the irradiated cells (conditioned media) was used to culture immune cells (THP-1 cells or Jurkat cells). Analyses were performed based on the response of immune cells in conditioned media. Sprague-Dawley rats received WBI at different doses, and were fed for one week to one month postirradiation. Spleen and peripheral blood were then isolated and analyzed. We observed that the number of monocytes in peripheral blood, and the level of NK cells and NKT cells in spleen increased after CNS injury. However, the level of T cells in spleen did not change and the level of B cells in the spleen decreased after γ-ray-induced CNS injury. These findings indicate that CNS injury caused by ionizing radiation induces a series of changes in the peripheral immune system.
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1 October 2019
Research Article|
October 01 2019
The Effect of Gamma-Ray-Induced Central Nervous System Injury on Peripheral Immune Response: An In Vitro and In Vivo Study
Peng Zhang;
Peng Zhang
1
a School of Life Science, Beijing Institute of Technology, Beijing, 100081, China
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Yu Chen;
Yu Chen
1
a School of Life Science, Beijing Institute of Technology, Beijing, 100081, China
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Huiyang Zhu;
Huiyang Zhu
a School of Life Science, Beijing Institute of Technology, Beijing, 100081, China
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Liben Yan;
Liben Yan
a School of Life Science, Beijing Institute of Technology, Beijing, 100081, China
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Chunli Sun;
Chunli Sun
a School of Life Science, Beijing Institute of Technology, Beijing, 100081, China
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Sizhu Pei;
Sizhu Pei
a School of Life Science, Beijing Institute of Technology, Beijing, 100081, China
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Adil Farooq Lodhi;
Adil Farooq Lodhi
a School of Life Science, Beijing Institute of Technology, Beijing, 100081, China
b Department of Microbiology, Faculty of Health Sciences, Hazara University, Mansehra, Pakistan
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Hao Ren;
Hao Ren
a School of Life Science, Beijing Institute of Technology, Beijing, 100081, China
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Yanan Gao;
Yanan Gao
a School of Life Science, Beijing Institute of Technology, Beijing, 100081, China
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Robina Manzoor;
Robina Manzoor
a School of Life Science, Beijing Institute of Technology, Beijing, 100081, China
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Bo Li;
Bo Li
a School of Life Science, Beijing Institute of Technology, Beijing, 100081, China
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Yulin Deng;
Yulin Deng
a School of Life Science, Beijing Institute of Technology, Beijing, 100081, China
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Hong Ma
Hong Ma
2
a School of Life Science, Beijing Institute of Technology, Beijing, 100081, China
2 Address for correspondence: Beijing Institute of Technology, School of Life Sciences, 5 Zhongguncun Street, Street 2, Beijing, Haidian 100081, China; email address: 04656@bit.edu.cn.
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Radiat Res (2019) 192 (4): 440–450.
Article history
Received:
March 08 2019
Accepted:
July 09 2019
Citation
Peng Zhang, Yu Chen, Huiyang Zhu, Liben Yan, Chunli Sun, Sizhu Pei, Adil Farooq Lodhi, Hao Ren, Yanan Gao, Robina Manzoor, Bo Li, Yulin Deng, Hong Ma; The Effect of Gamma-Ray-Induced Central Nervous System Injury on Peripheral Immune Response: An In Vitro and In Vivo Study. Radiat Res 1 October 2019; 192 (4): 440–450. doi: https://doi.org/10.1667/RR15378.1
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