Cold inducible RNA binding protein (CIRP), also named A18 hnRNP or CIRBP, is a cold-shock RNA-binding protein which can be induced upon various cellular stresses. Its expression level is induced in various cancer tissues relative to adjacent normal tissues; this is believed to play a critical role in cancer development and progression. In this study, we investigated the role of CIRP in cells exposed to ionizing radiation. Our data show that CIRP reduction causes cell colony formation and cell viability reduction after irradiation. In addition, CIRP knockdown cells demonstrated a higher DNA damage rate but less cell cycle arrest after irradiation. As a result, the induced DNA damage with less DNA repair processes led to an increased cell apoptosis rate in CIRP knockdown cells postirradiation. These findings suggest that CIRP is a critical protein in irradiated cells and can be used as a potential target for sensitizing cancer cells to radiation therapy.
Skip Nav Destination
REGULAR ARTICLES| November 12 2020
CIRP Sensitizes Cancer Cell Responses to Ionizing Radiation
Adele P. Bergmeier;
Lingying Tong 1
a Edison Biotechnology Institute, Ohio University, Athens, Ohio 45701
c Department of Chemistry and Biochemistry, Ohio University, Ohio 45701
1 Address for correspondence: Edison Biotechnology Institute, 101 Konneker Laboratories, The Ridges, Building 25, Athens, OH 45701; email: firstname.lastname@example.org.
Search for other works by this author on:
Weichao Sun, Adele P. Bergmeier, Yi Liao, Shiyong Wu, Lingying Tong; CIRP Sensitizes Cancer Cell Responses to Ionizing Radiation. Radiat Res 1 January 2021; 195 (1): 93–100. doi: https://doi.org/10.1667/RADE-20-00063.1
Download citation file:
Citing articles via
Commonalities Between COVID-19 and Radiation Injury
Carmen I. Rios, David R. Cassatt, Brynn A. Hollingsworth, Merriline M. Satyamitra, Yeabsera S. Tadesse, Lanyn P. Taliaferro, Thomas A. Winters, Andrea L. DiCarlo
Effects of Radiation on Blood Pressure and Body Weight in the Spontaneously Hypertensive Rat Model. Are Radiation Effects on Blood Pressure Affected by Genetic Background?
Norio Takahashi, Munechika Misumi, Yasuharu Niwa, Hideko Murakami, Waka Ohishi, Toshiya Inaba, Akiko Nagamachi, Satoshi Tanaka, Ignacia Braga Tanaka, III, Gen Suzuki
Studies of the Mortality of Atomic Bomb Survivors, Report 14, 1950–2003: An Overview of Cancer and Noncancer Diseases
Kotaro Ozasa, Yukiko Shimizu, Akihiko Suyama, Fumiyoshi Kasagi, Midori Soda, Eric J. Grant, Ritsu Sakata, Hiromi Sugiyama, Kazunori Kodama
Long-Term Effects of the Rain Exposure Shortly after the Atomic Bombings in Hiroshima and Nagasaki
Ritsu Sakata, Eric J. Grant, Kyoji Furukawa, Munechika Misumi, Harry Cullings, Kotaro Ozasa, Roy E. Shore
Radiofrequency Fields and Calcium Movements Into and Out of Cells
Andrew Wood, Ken Karipidis