Human papillomavirus (HPV) infection is strongly associated with cervical cancer (CC). Genomic alterations caused by viral infection and subsequent dysregulation of cellular metabolism under hypoxic conditions could influence the response to treatment. We studied a possible influence of IGF-1Rb, hTERT, HIF1a, GLUT1 protein expression, HPV species presence and relevant clinical parameters on the response to treatment. In 21 patients, HPV infection and protein expression were detected using GP5+/GP6+PCR-RLB and immunohistochemistry, respectively. The worse response was associated with radiotherapy alone compared with chemoradiotherapy (CTX-RT), anemia and HIF1a expression. HPV16 type was the most frequent (57.1%) followed by HPV-58 (14.2%) and HPV-56 (9.5%). The HPV alpha 9 species was the most frequent (76.1%) followed by alpha 6 and alpha 7. IGF-1Rb (85.7%), HIF1a (61.9%), GLUT1 (52.3%), and hTERT expression [cytoplasm and nucleus (90.4%)] were detected. The MCA factorial map showed different relationships, standing out, expression of hTERT and alpha 9 species HPV, expression of hTERT and IGF-1Rb expression [Fisher's exact test (P = 0.04)]. A slight trend of association was observed between, GLUT1 and HIF1 a expression, hTERT and GLUT1 expression. A noteworthy finding was the subcellular localization of hTERT in the nucleus and cytoplasm of CC cells and its possible interaction with IGF-1R in presence of HPV alpha 9 species. Our findings suggest that the expression of HIF1a, hTERT, IGF-1Rb and GLUT1 proteins that interact with some HPV species may contribute to cervical cancer development, and the modu lation of treatment response.
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RESEARCH ARTICLES|
March 07 2023
hTERT and IGF-1R Proteins Expression in Response to Treatment in Patients with HPV Alpha 9-Positive Cervical Cancer
Pablo Moreno-Acosta;
Pablo Moreno-Acosta
1
a Cancer Biology Research Group, Instituto Nacional de Cancerología, Calle 1 # 9-85, Bogotá, Colombia
b Clinical, Molecular and Cellular Radiobiology Research Group, Instituto Nacional de Cancerología, Calle 1 # 9-85, Bogotá, Colombia
1 Correspondence to: Dr. P. Moreno Acosta, Instituto Nacional de Cancerología, Cancer Biology Research Group, Clinical, Molecular and Cellular Radiobiology Research Group, Research, Calle 1ª No 9-85; Bogotá, Colombia: e-mail: [email protected].
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Oscar Gamboa;
Oscar Gamboa
b Clinical, Molecular and Cellular Radiobiology Research Group, Instituto Nacional de Cancerología, Calle 1 # 9-85, Bogotá, Colombia
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Cristian González-Prieto;
Cristian González-Prieto
c School of Computer Science, The University of Auckland, Auckland, New Zealand
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Alfredo Romero-Rojas;
Alfredo Romero-Rojas
d Oncological Pathology Group, Instituto Nacional de Cancerología de Colombia, Calle 1 # 9-85, Bogotá, Colombia
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Josep Balart Serra;
Josep Balart Serra
e Servei d'Oncologia Radioteràpica, Hospital de la Santa Creu i Sant Pau, Barcelona, España. Carrer de Sant Quintí, 89, 08026 Barcelona
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German Dario Díaz;
German Dario Díaz
b Clinical, Molecular and Cellular Radiobiology Research Group, Instituto Nacional de Cancerología, Calle 1 # 9-85, Bogotá, Colombia
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Gina Malaver;
Gina Malaver
a Cancer Biology Research Group, Instituto Nacional de Cancerología, Calle 1 # 9-85, Bogotá, Colombia
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Wafa Bouleftour;
Wafa Bouleftour
f Department of Radiotherapy, North Hospital, University Hospital of Saint-Etienne, Saint-Etienne, France; Saint Etienne Medicine University, Saint Etienne, France
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Nicolas Magné
Nicolas Magné
b Clinical, Molecular and Cellular Radiobiology Research Group, Instituto Nacional de Cancerología, Calle 1 # 9-85, Bogotá, Colombia
f Department of Radiotherapy, North Hospital, University Hospital of Saint-Etienne, Saint-Etienne, France; Saint Etienne Medicine University, Saint Etienne, France
g Department of Radiation Oncology, Institut Bergonié, Bordeaux, France
h Cellular and Molecular Radiobiology laboratory, CNRS UMR 5822, Institut de Physique Nucléaire de Lyon, IPNL; Lyon Medicine University, Lyon, France
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Radiat Res (2023) 199 (5): 452–459.
Article history
Received:
July 11 2022
Accepted:
February 22 2023
Citation
Pablo Moreno-Acosta, Oscar Gamboa, Cristian González-Prieto, Alfredo Romero-Rojas, Josep Balart Serra, German Dario Díaz, Gina Malaver, Wafa Bouleftour, Nicolas Magné; hTERT and IGF-1R Proteins Expression in Response to Treatment in Patients with HPV Alpha 9-Positive Cervical Cancer. Radiat Res 1 May 2023; 199 (5): 452–459. doi: https://doi.org/10.1667/RADE-22-00121.1
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