Radiotherapy is a common therapeutic strategy for various solid tumors, with vascular endothelial injury being a common side effect. The study aimed to examine the effect of long non-coding RNA PVT1 on radiation-induced vascular endothelial cell injury, and explore the possible underlying mechanism. Human umbilical vein endothelial cells (HUVECs) were exposed to different doses of X ray to mimic radiation. LncRNA and miRNA levels were detected via qRT-PCR. Interaction between lncRNA and miRNAs was determined through dual-luciferase reporter assay. Statistical processing was conducted using student’s t test between two groups and one-way ANOVA among multiple groups, and P < 0.05 means a significant difference. GO and KEGG were performed for the function and pathway enrichment analysis. LncRNA PVT1 elevated along with the increase of radiation dose in HUVECs. Poorly expressed lncRNA PVT1 promotes cell viability and inhibits oxidative stress. PVT1 serves as a competitive endogenous RNA (ceRNA) of miR-9-5p. miR-9-5p inhibitor inverted the influence of PVT1 knockdown on radiation-stimulated cell apoptosis and oxidative stress in HUVECs. KEGG analysis identified significant enrichment of the MAPK signaling pathway among overlapping target genes of miR-9-5p. LncRNA PVT1 knockdown alleviated radiation-induced vascular endothelial injury via sponging miR-9-5p. The underlying mechanism might be probably MAPK signaling-related.
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October 2024
REGULAR ARTICLES|
October 08 2024
Long Non-Coding RNA PVT1 Facilitates Radiation-Induced Vascular Endothelial Cell Injury through Sponging MicroRNA-9-5p
Jing Wang;
Jing Wang
1
aDepartment of Imaging, The Second Clinical School of North Sichuan Medical College/Nanchong Central Hospital, Nanchong 637000, China
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Yanting Zhang;
Yanting Zhang
1
bDepartment of Cardiology, Yichang Hospital of Traditional Chinese Medicine, Yichang 443000, China
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Wei Lian;
Wei Lian
cDepartment of Imaging, Yancheng Third People’s Hospital, Yancheng 224001, China
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Min Gan
Min Gan
2
cDepartment of Imaging, Yancheng Third People’s Hospital, Yancheng 224001, China
2Corresponding author: Min Gan, Department of Imaging, Yancheng Third People’s Hospital, No.75, Theater Road, Tinghu District, Yancheng, 224001, Jiangsu, China; email: [email protected].
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Radiat Res (2024) 202 (4): 670–676.
Article history
Received:
March 18 2024
Accepted:
July 08 2024
Citation
Jing Wang, Yanting Zhang, Wei Lian, Min Gan; Long Non-Coding RNA PVT1 Facilitates Radiation-Induced Vascular Endothelial Cell Injury through Sponging MicroRNA-9-5p. Radiat Res 1 October 2024; 202 (4): 670–676. doi: https://doi.org/10.1667/RADE-24-00089.1
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