The radiosensitivity of mice differs between postnatal days 10 (P10) and 21(P21); these days mark different stages of brain development. In the present study, Ki67 and doublecotin (DCX) immunostaining and hematoxylin staining was performed, which showed that acute radiation exposure at postnatal day 10 induced higher cell apoptosis and loss in the hilus of the dentate gyrus at day 1 postirradiation than postnatal day 21. MicroRNA (miRNA) sequencing and real-time quantitative reverse transcription PCR (qRT-PCR) analysis indicated the upregulation of miRNA-34a-5p at days 1 and 7 after irradiation at postnatal day 10, but not at postnatal day 21. Down-regulation of T-cell intracytoplasmic antigen-1 pathway (Tia1) was indicated by qRT-PCR at day 1 day but not day 7 after irradiation at postnatal day 10. Neurobehavioral testing in mature mice irradiated at postnatal day 10 demonstrated the impairment of short-term memory in novel object recognition and spatial memory, compared to those irradiated at postnatal day 21. Combined with our previous luciferase assay showing the direct interaction of miRNA34a-5p and Tia1, these findings suggest that radiation-induced abnormal miR-34a-5p/Tial interaction at day 1 after irradiation at postnatal day 10 may be involved in apoptosis of the dentate gyrus hilar, impairment of neurogenesis and subsequent short-term memory loss as observed in the novel object recognition and Barnes maze tests.
Skip Nav Destination
Article navigation
October 2024
REGULAR ARTICLES|
October 08 2024
Radiosensitivity-related Variation in MicroRNA-34a-5p Levels and Subsequent Neuronal Loss in the Hilus of the Dentate Gyrus after Irradiation at Postnatal Days 10 and 21 in Mice
Lian Liu;
Lian Liu
aThe School of Basic Medicine, Health Science Center, Yangtze University, Jingzhou, Hubei 434023, China
Search for other works by this author on:
Hong Wang;
Hong Wang
bRadiation Physiology Laboratory, Singapore Nuclear Research and Safety Initiative, National University of Singapore, 138602, Singapore
Search for other works by this author on:
Zhao Wu Ma;
Zhao Wu Ma
aThe School of Basic Medicine, Health Science Center, Yangtze University, Jingzhou, Hubei 434023, China
Search for other works by this author on:
Feng Ru Tang
Feng Ru Tang
1
bRadiation Physiology Laboratory, Singapore Nuclear Research and Safety Initiative, National University of Singapore, 138602, Singapore
1Address for correspondence: Dr. Feng Ru Tang; Radiation Physiology Laboratory, Singapore Nuclear Research and Safety Initiative, National University of Singapore, 138602, Singapore; email: [email protected].
Search for other works by this author on:
Radiat Res (2024) 202 (4): 677–684.
Article history
Received:
December 01 2023
Accepted:
July 08 2024
Citation
Lian Liu, Hong Wang, Zhao Wu Ma, Feng Ru Tang; Radiosensitivity-related Variation in MicroRNA-34a-5p Levels and Subsequent Neuronal Loss in the Hilus of the Dentate Gyrus after Irradiation at Postnatal Days 10 and 21 in Mice. Radiat Res 1 October 2024; 202 (4): 677–684. doi: https://doi.org/10.1667/RADE-23-00248.1
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionCiting articles via
Dosimetry: Was and Is an Absolute Requirement for Quality Radiation Research
Daniel Johnson, H. Harold Li, Bruce F. Kimler
Cutaneous Radiation Injuries: Models, Assessment and Treatments
Andrea L. DiCarlo, Aaron C. Bandremer, Brynn A. Hollingsworth, Suhail Kasim, Adebayo Laniyonu, Nushin F. Todd, Sue-Jane Wang, Ellen R. Wertheimer, Carmen I. Rios
Effects of Radiation on Blood Pressure and Body Weight in the Spontaneously Hypertensive Rat Model. Are Radiation Effects on Blood Pressure Affected by Genetic Background?
Norio Takahashi, Munechika Misumi, Yasuharu Niwa, Hideko Murakami, Waka Ohishi, Toshiya Inaba, Akiko Nagamachi, Satoshi Tanaka, Ignacia Braga Tanaka, III, Gen Suzuki
Studies of the Mortality of Atomic Bomb Survivors, Report 14, 1950–2003: An Overview of Cancer and Noncancer Diseases
Kotaro Ozasa, Yukiko Shimizu, Akihiko Suyama, Fumiyoshi Kasagi, Midori Soda, Eric J. Grant, Ritsu Sakata, Hiromi Sugiyama, Kazunori Kodama
The Technical and Clinical Implementation of LATTICE Radiation Therapy (LRT)
Xiaodong Wu, Naipy C. Perez, Yi Zheng, Xiaobo Li, Liuqing Jiang, Beatriz E. Amendola, Benhua Xu, Nina A. Mayr, Jiade J. Lu, Georges F. Hatoum, Hualin Zhang, Sha X. Chang, Robert J. Griffin, Chandan Guha