Sarcoma 180 and Ehrlich ascites tumor cells were irradiated in vitro as a 1:10 or 1:1000 suspension in normal saline. During irradiation, metabolism was inhibited by maintaining the cells at 0°C. Dose-effect curves for survival and reproduction were determined during the exponential growth phase (9 days) after intraperitoneal inoculation of approximately 4000 cells. Cells irradiated in low oxygen environment suffered more injury in the diluted (1:1000) than in the more concentrated (1:10) suspension. The results at 9 days were confirmed in their general aspects by ultimate survival (percentage of tumor takes). Dilution of the cell population during exposure was found to be a factor that was distinguishable from that of the oxygen effect.
Effect of Cell Concentration during Irradiation at Low Oxygen Tension on Survival of Mouse Ascites Tumor Cells
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T. C. Evans, R. F. Hagemann, D. B. Leeper; Effect of Cell Concentration during Irradiation at Low Oxygen Tension on Survival of Mouse Ascites Tumor Cells. Radiat Res 1 July 1968; 35 (1): 123–131. doi: https://doi.org/10.2307/3572439
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