A method which combined the principles of the "window" technique and the double emulsion radioautographic study of14 C- and3 H-labeled cells was developed to study DNA synthesis of mouse leukemic cells as they passed through the S period. With this method, it was shown that the radiation caused a depression of DNA synthesis followed by the recovery of DNA synthesis to a rate that was faster than that of nonirradiated cells. The irradiated cells were able to synthesize more DNA than the nonirradiated control cells. A DNA degradation process similar to that in bacteria was not detected in the lethally irradiated cells. These findings were discussed in relation to cell death.
Deoxyribonucleic Acid Synthesis during the First Postirradiation Life Cycle of Lethally Irradiated Cultured Mammalian Cells (L5178Y)
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I. Watanabe, S. Okada; Deoxyribonucleic Acid Synthesis during the First Postirradiation Life Cycle of Lethally Irradiated Cultured Mammalian Cells (L5178Y). Radiat Res 1 July 1968; 35 (1): 202–212. doi: https://doi.org/10.2307/3572445
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