The radiosensitizing effect of triacetoneamine N-oxyl (TAN) on anoxic suspensions of two x-ray-sensitive mutants of E. coli K12 was investigated. With one mutant, AB2463 recA13, TAN increased the sensitivity (based on the dose required to give 1% surviving fraction) of anoxic cells by a factor of 3.9, while oxygen increased the sensitivity by a factor of only 1.9. On the other hand, on the same basis, TAN was only about one-half as effective as oxygen in sensitizing the mutant AB3058 rec-22. The results are interpreted in terms of the relative ability of the cells to recover from radiation damage produced in the presence of TAN and in the presence of oxygen.

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