The effects of whole-body gamma irradiation, which blocks DNA synthesis, and puromycin, a protein synthesis inhibitor, on total organ activities of three acid hydrolases of regenerating, radiosensitive rat liver, were studied. Doses of 1000 or 10,000 rads, given immediately or 6 hours after subtotal hepatectomy, failed to prevent the twofold increase in total organ activities normally elicited by partial hepatectomy. Puromycin, injected every 3 hours in amounts large enough to inhibit protein synthesis, was also not effective. The observed rise of total organ hydrolase activities in regenerating liver may be due to selective removal of natural inhibitors of the enzymes, or to some form of enzyme activation. The process is not sensitive to gamma irradiation or protein synthesis arrest.

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