The effect of liquid-holding after γ and uv irradiation was investigated in three different strains of Schizosaccharomyces pombe: the haploid wild type <tex-math>$972\ {\rm h}^{-}$</tex-math>, the diploid <tex-math>${\rm h}^{-}/{\rm h}^{-}$</tex-math> and the radiation-sensitive strain UVS-1 <tex-math>${\rm h}^{-}$</tex-math>. It was found that the diploid strain is only slightly more resistant to inactivation by both γ and uv irradiation compared to the haploid wild type. For liquid-holding experiments various non-nutrient solutions were examined and two which yielded minimal spontaneous cell death were chosen as favorable for all subsequent studies. These experiments have shown that: (i) the haploid wild type exhibited negative liquid-holding effect (NLHE) after γ irradiation, similar to that reported previously for uv irradiation; (ii) the diploid strain showed NLHE after both γ and uv irradiation; the rate of NLHE, for a given dose, was similar to that for the haploid strain; (iii) for both haploid and diploid strains the rate of NLHE increased with increasing dose of either γ or uv irradiation; (iv) the radiation-sensitive strain UVS-1 <tex-math>${\rm h}^{-}$</tex-math>, for which liquid-holding recovery (LHR) following uv irradiation has been reported, did not display LHR after γ irradiation; (v) the pH of the two non-nutrient solutions used (pH 4.8 and 7.0), had no effect on the LH response of any of the three strains.

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Copyright 1973 Academic Press, Inc.
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