Gamma irradiation of dormant barley seeds has led to the production of DNA single-strand breaks, the liberation of inorganic phosphate, and the formation of phosphomonoester groups. For each single-strnad break produced in DNA, 0.22 molecules of inorganic phosphate were liberated and 1.1 molecules of phosphomonoester groups were formed. Of the phosphomonoester groups formed, 40% were 5′-phosphomonoesters. Only 8.6% of the 5′-termini produced by radiation were 5′-hydroxyl termini. The chemical mechanisms involved in the induction of single-strand breaks in the DNA of dormant barley seeds are discussed.
Chemical Nature of Radiation-Induced Single-Strand Breaks in the DNA of Dormant Barley Seeds in Vivo
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Ahmed El-Metainy, Shigemitsu Tano, Keiji Yano, Hikoyuki Yamaguchi; Chemical Nature of Radiation-Induced Single-Strand Breaks in the DNA of Dormant Barley Seeds in Vivo. Radiat Res 1 August 1973; 55 (2): 324–333. doi: https://doi.org/10.2307/3573687
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