The separate contributions of the primary radical species to changes in enzymatic activity, amino acid composition and molecular configuration of RNase S-peptide have been determined by γ-irradiation of dilute solutions in controlled environments. For H· and ·OH, approximately one out of two radicals is effective for the enzymatic inactivation of S-peptide; <tex-math>$e^{-}{}_{{\rm aq}}$</tex-math> is much less effective, only one out of eight or more radicals causing loss of activity. Amino acid analyses of irradiated S-peptide show that only the single sulfur-containing residue, methionine, and the single aromatic residue, phenylalanine, are significantly destroyed. For H atom attack, loss of enzymatic activity correlates with the destruction of methionine; phenylalanine is much less affected and no new products are separated on gel filtration. Hydroxyl radicals cause a significant destruction of phenylalanine, as well as methionine, and gel filtration shows that a new product of higher molecular weight is formed.
Skip Nav Destination
Close
Article navigation
1 December 1974
Research Article|
December 01 1974
Inactivation of Ribonuclease S-Peptide by the Primary Aqueous Radicals
Radiat Res (1974) 60 (3): 422–431.
Citation
L. K. Mee, S. J. Adelstein; Inactivation of Ribonuclease S-Peptide by the Primary Aqueous Radicals. Radiat Res 1 December 1974; 60 (3): 422–431. doi: https://doi.org/10.2307/3574022
Download citation file:
Close
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Sign in via your Institution
Sign in via your InstitutionCiting articles via
Commonalities Between COVID-19 and Radiation Injury
Carmen I. Rios, David R. Cassatt, Brynn A. Hollingsworth, Merriline M. Satyamitra, Yeabsera S. Tadesse, Lanyn P. Taliaferro, Thomas A. Winters, Andrea L. DiCarlo
Low-Dose Radiation Therapy (LDRT) for COVID-19: Benefits or Risks?
Pataje G. Prasanna, Gayle E. Woloschak, Andrea L. DiCarlo, Jeffrey C. Buchsbaum, Dörthe Schaue, Arnab Chakravarti, Francis A. Cucinotta, Silvia C. Formenti, Chandan Guha, Dale J. Hu, Mohammad K. Khan, David G. Kirsch, Sunil Krishnan, Wolfgang W. Leitner, Brian Marples, William McBride, Minesh P. Mehta, Shahin Rafii, Elad Sharon, Julie M. Sullivan, Ralph R. Weichselbaum, Mansoor M. Ahmed, Bhadrasain Vikram, C. Norman Coleman, Kathryn D. Held
Germicidal Efficacy and Mammalian Skin Safety of 222-nm UV Light
Manuela Buonanno, Brian Ponnaiya, David Welch, Milda Stanislauskas, Gerhard Randers-Pehrson, Lubomir Smilenov, Franklin D. Lowy, David M. Owens, David J. Brenner