A procedure is described for preparing lambda phage labeled in either strand r or l with radioisotopes, 5-bromouracil, or both. Phage containing 5-bromouracil in strand r have the same photosensitivity to near-visible light as phage containing 5-bromouracil in strand l. Lesions in both types of phage DNA can be repaired to the same extent by the bacterial DNA excision repair system.

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