DNA single-strand-breakage efficiency was measured in frozen Chinese hamster cells (V79-S171) after they accumulated disintegrations from incorporated tritium. The results are compared to the single-strand-breakage efficiency of X-rays at dry ice or liquid nitrogen temperatures, which is reduced by a factor of 4 from the strand-breakage efficiency found at 37°C.3 H decays in cells that incorporated [5-3 H]uridine into RNA after a 30-min pulse were 20% as effective as those in [3 H]thymidine-labeled cells in causing single-strand breaks in DNA. This difference in DNA strand-breakage efficiencies for [3 H]uridine and [3 H]thymidine is almost the same as the difference in efficiencies of cell killing from the two macromolecular locations of tritium within the nucleus of the cell. In cells labeled in one or both strands of DNA with [3 H]thymidine the breakage efficiencies were 2.2-2.3 breaks per3 H decay, equivalent to 0.5 rads per decay-a value very close to that predicted for the dosimetry of3 H beta particles. Irradiation by3 H decays from tritiated water added to the medium before freezing was intermediate between [3 H] uridine and [3 H]thymidine in producing single-strand breaks. Isolation of3 H-labeled DNA and freezing in solution at a concentration less than that found in the cell nucleus produced a lower strand-breakage efficiency because of a reduction in the dose to the DNA by the short-ranged beta particles.
Skip Nav Destination
Research Article| May 01 1975
DNA Damage from Incorporated Radioisotopes: Influence of the3 H Location in the Cell
H. John Burki ;
Stephen Bunker ;
Radiat Res (1975) 62 (2): 299–312.
- Views Icon Views
- Share Icon Share
- Search Site
H. John Burki, Stephen Bunker, Mark Ritter, James E. Cleaver; DNA Damage from Incorporated Radioisotopes: Influence of the3 H Location in the Cell. Radiat Res 1 May 1975; 62 (2): 299–312. doi: https://doi.org/10.2307/3574222
Download citation file:
Citing articles via
Commonalities Between COVID-19 and Radiation Injury
Carmen I. Rios, David R. Cassatt, Brynn A. Hollingsworth, Merriline M. Satyamitra, Yeabsera S. Tadesse, Lanyn P. Taliaferro, Thomas A. Winters, Andrea L. DiCarlo
Low-Dose Radiation Therapy (LDRT) for COVID-19: Benefits or Risks?
Pataje G. Prasanna, Gayle E. Woloschak, Andrea L. DiCarlo, Jeffrey C. Buchsbaum, Dörthe Schaue, Arnab Chakravarti, Francis A. Cucinotta, Silvia C. Formenti, Chandan Guha, Dale J. Hu, Mohammad K. Khan, David G. Kirsch, Sunil Krishnan, Wolfgang W. Leitner, Brian Marples, William McBride, Minesh P. Mehta, Shahin Rafii, Elad Sharon, Julie M. Sullivan, Ralph R. Weichselbaum, Mansoor M. Ahmed, Bhadrasain Vikram, C. Norman Coleman, Kathryn D. Held
Germicidal Efficacy and Mammalian Skin Safety of 222-nm UV Light
Manuela Buonanno, Brian Ponnaiya, David Welch, Milda Stanislauskas, Gerhard Randers-Pehrson, Lubomir Smilenov, Franklin D. Lowy, David M. Owens, David J. Brenner