The effect of osmium tetroxide oxidation of single-stranded, circular φX174-DNA on its infectivity in E. coli spheroplasts was investigated. Osmium tetroxide predominantly oxidizes thymine to 5,6-dihydroxy-dihydrothymine in DNA and causes only negligible amounts of strand breakage. Products of the 5,6-dihydroxy-dihydrothymine type represent typical lesions introduced into DNA by ionizing radiation. It was found that osmium tetroxide oxidation inactivated the DNA and that at 37% residual infectivity the φX174-genome contained on average 2.8 ring-saturated thymine residues. No significant differences were observed for spheroplasts from wild type E. coli, E. coli polA, E. coli uvrA, E. coli recA, and E. coli recB.
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Research Article| February 01 1977
Biological Effect of Thymine Ring Saturation in Coliphage φX174-DNA
P. V. Hariharan ;
Radiat Res (1977) 69 (2): 375–378.
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P. V. Hariharan, P. M. Achey, P. A. Cerutti; Biological Effect of Thymine Ring Saturation in Coliphage φX174-DNA. Radiat Res 1 February 1977; 69 (2): 375–378. doi: https://doi.org/10.2307/3574445
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