We have studied the effects of γ irradiation at different temperatures on the hydration water proton resonance spectra from frozen${\rm H}_{2}{\rm O}/{\rm D}_{2}{\rm O}$ solutions of DNA and polynucleotides. Comparison of results obtained with pure H2 O solutions showed a striking difference in the temperature-dependent variations of$\Delta _{{\textstyle\frac{1}{2}}}$ values in nonirradiated and irradiated DNA solutions. There is evidence that base rehydration with hydration water molecules occurs after an initial damage to the macromolecular chains in DNA caused by the direct or indirect radiation effects.

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