DNA repair has been investigated, estimated by unscheduled DNA synthesis (UDS) and the cellular NAD+ pool, after exposing human mononuclear leukocytes to hyperthermia and γ radiation separately and in combination. It was found that γ radiation induced a decline in UDS with increasing temperature through the temperature region studied (37-45°C). At 42.5°C the γ-ray-induced UDS was reduced to about 70% of that at 37°C. Following γ-ray damage the NAD+ pool dropped to about 20% of control values. Without hyperthermic treatment the cells completely recovered to the original level within 5 hr. Moderate hyperthermia (42.5°C for 45 min) followed by γ-ray damage altered the kinetics so that even after 8 hr the NAD+ pool had recovered to only 70% of the original level. After heat treatment at 44°C for 45 min prior to γ radiation the cells did not recover at all, presumably because of the cytotoxic effects from the combined treatment.
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1 January 1984
Research Article|
January 01 1984
Effects of γ Radiation and Hyperthermia on DNA Repair Synthesis and the Level of NAD+ in Cultured Human Mononuclear Leukocytes
Radiat Res (1984) 97 (1): 97–107.
Citation
Göran G. Jonsson, Göran Eriksson, Ronald W. Pero; Effects of γ Radiation and Hyperthermia on DNA Repair Synthesis and the Level of NAD+ in Cultured Human Mononuclear Leukocytes. Radiat Res 1 January 1984; 97 (1): 97–107. doi: https://doi.org/10.2307/3576192
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