Bone fragility is a well-documented long-term side effect of radiotherapy, which currently has no preventative treatments. In this study, we applied a caloric restriction (CR) diet to attenuate both local and systemic bone loss after irradiation (RTx) in an established female Balb/c mouse model (4 consecutive daily doses of 5 Gy to the right hindlimb only). CR mice were tapered down to a 30% reduced calorie diet (RTx/CR) one week before irradiation, while regular diet (RD) mice received food ad libitum (RTx/RD). Unirradiated (sham) mice received either a 30% CR diet (SH/CR) or received food ad libitum (SH/RD). Irradiated, contralateral, and unirradiated hindlimbs were evaluated at 2, 4, and 8 weeks postirradiation using micro-computed tomography (μCT) to assess bone morphology and 3-point bending to quantify femur strength. Histological analysis of irradiated and unirradiated tibiae was performed to examine general bone tissue cytology and serum biomarker analysis was performed using terminal blood draw samples. After treatment, femur strength and metaphyseal bone quantity was decreased in irradiated and contralateral femora of RTx/RD mice compared to SH/RD femurs; this finding is consistent with previous studies. RTx/CR mice had positive effects when compared to RTx/RD mice, including increased strength relative to body mass in both the irradiated and contralateral limb, increased trabecular bone mass, and decreased marrow adiposity. However, a number of adverse effects were also observed, including a significant decrease in body mass and decreased cortical bone. Overall, CR shows promise as a preventative treatment for postirradiated bone fragility, yet questions remain to be addressed in future studies. Ideal diet duration, impact to normal tissue, and mechanism of action must be explored to better understand the clinical implication of a CR diet.
Skip Nav Destination
Article navigation
Research Article|
September 23 2024
Caloric Restriction Diet Attenuates Systemic Bone Fragility after Radiotherapy
Jessica A. Stering;
Jessica A. Stering
aDepartment of Orthopedic Surgery, SUNY Upstate Medical University, Syracuse, New York
Search for other works by this author on:
Amy E. Biggs;
Amy E. Biggs
aDepartment of Orthopedic Surgery, SUNY Upstate Medical University, Syracuse, New York
Search for other works by this author on:
Tara E. Carney;
Tara E. Carney
aDepartment of Orthopedic Surgery, SUNY Upstate Medical University, Syracuse, New York
Search for other works by this author on:
Megan E. Oest;
Megan E. Oest
1
aDepartment of Orthopedic Surgery, SUNY Upstate Medical University, Syracuse, New York
1Corresponding author: Megan E. Oest, Associate Professor, Department of Orthopedic Surgery, SUNY Upstate Medical University, 750 East Adams Street, Syracuse, New York, 13210; email: [email protected]; Phone: 315-464-9950.
Search for other works by this author on:
Brittany A. Simone
Brittany A. Simone
bDepartment of Radiation Oncology, SUNY Upstate Medical University, Syracuse, New York
Search for other works by this author on:
Radiat Res (2024)
Article history
Received:
November 03 2023
Accepted:
September 04 2024
Citation
Jessica A. Stering, Amy E. Biggs, Tara E. Carney, Megan E. Oest, Brittany A. Simone; Caloric Restriction Diet Attenuates Systemic Bone Fragility after Radiotherapy. Radiat Res 2024; doi: https://doi.org/10.1667/RADE-23-00227.1
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your Institution
2
Views
Citing articles via
Dosimetry: Was and Is an Absolute Requirement for Quality Radiation Research
Daniel Johnson, H. Harold Li, Bruce F. Kimler
Exposure to Ionizing Radiation and Risk of Dementia: A Systematic Review and Meta-Analysis
Tanvi Srivastava, Ekaterina Chirikova, Sapriya Birk, Fanxiu Xiong, Tarek Benzouak, Jane Y. Liu, Paul J. Villeneuve, Lydia B. Zablotska
Effects of Radiation on Blood Pressure and Body Weight in the Spontaneously Hypertensive Rat Model. Are Radiation Effects on Blood Pressure Affected by Genetic Background?
Norio Takahashi, Munechika Misumi, Yasuharu Niwa, Hideko Murakami, Waka Ohishi, Toshiya Inaba, Akiko Nagamachi, Satoshi Tanaka, Ignacia Braga Tanaka, III, Gen Suzuki
Space Radiation and Human Exposures, A Primer
Gregory A. Nelson
Cutaneous Radiation Injuries: Models, Assessment and Treatments
Andrea L. DiCarlo, Aaron C. Bandremer, Brynn A. Hollingsworth, Suhail Kasim, Adebayo Laniyonu, Nushin F. Todd, Sue-Jane Wang, Ellen R. Wertheimer, Carmen I. Rios