To identify modifications to amino acids that are directly induced by ionizing radiation, free amino acids and 3-residue peptides were irradiated using a linear accelerator (Linac) radiotherapy device. Mass spectrometry was performed to detail the relative sensitivity to radiation as well as identify covalent, radiation-dependent adducts. The order of reactivity of the 20 common amino acids was generally in agreement with published literature except for His (most reactive of the 20) and Cys (less reactive). Novel and previously identified modifications on the free amino acids were detected. Amino acids were far less reactive when flanked by glycine residues in a tripeptide. Order of reactivity, with GVG most and GEG least, was substantially altered, as were patterns of modification. Radiation reactivity of amino acids is clearly and strongly affected by conversion of the α-amino and α-carboxyl groups to peptide bonds, and the presence of neighboring amino acid residues.
Skip Nav Destination
Article navigation
1 May 2019
REGULAR ARTICLES|
March 08 2019
Covalent Modification of Amino Acids and Peptides Induced by Ionizing Radiation from an Electron Beam Linear Accelerator Used in Radiotherapy
Benjamin B. Minkoff;
Benjamin B. Minkoff
aBiotechnology Center, University of Wisconsin-Madison, Madison, Wisconsin 53706
Search for other works by this author on:
Steven T. Bruckbauer;
Steven T. Bruckbauer
bDepartment of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706
Search for other works by this author on:
Grzegorz Sabat;
Grzegorz Sabat
aBiotechnology Center, University of Wisconsin-Madison, Madison, Wisconsin 53706
Search for other works by this author on:
Michael M. Cox;
Michael M. Cox
bDepartment of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706
Search for other works by this author on:
Michael R. Sussman
Michael R. Sussman
1
aBiotechnology Center, University of Wisconsin-Madison, Madison, Wisconsin 53706
1Address for correspondence: University of Wisconsin-Madison, Biochemistry and Biotechnology Center, 425 Henry Mall, Madison, WI 53706; email address: msussman@wisc.edu.
Search for other works by this author on:
Radiat Res (2019) 191 (5): 447–459.
Article history
Received:
November 12 2018
Accepted:
February 13 2019
Citation
Benjamin B. Minkoff, Steven T. Bruckbauer, Grzegorz Sabat, Michael M. Cox, Michael R. Sussman; Covalent Modification of Amino Acids and Peptides Induced by Ionizing Radiation from an Electron Beam Linear Accelerator Used in Radiotherapy. Radiat Res 1 May 2019; 191 (5): 447–459. doi: https://doi.org/10.1667/RR15288.1
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionCiting articles via
Commonalities Between COVID-19 and Radiation Injury
Carmen I. Rios, David R. Cassatt, Brynn A. Hollingsworth, Merriline M. Satyamitra, Yeabsera S. Tadesse, Lanyn P. Taliaferro, Thomas A. Winters, Andrea L. DiCarlo
Studies of the Mortality of Atomic Bomb Survivors, Report 14, 1950–2003: An Overview of Cancer and Noncancer Diseases
Kotaro Ozasa, Yukiko Shimizu, Akihiko Suyama, Fumiyoshi Kasagi, Midori Soda, Eric J. Grant, Ritsu Sakata, Hiromi Sugiyama, Kazunori Kodama
Effects of Radiation on Blood Pressure and Body Weight in the Spontaneously Hypertensive Rat Model. Are Radiation Effects on Blood Pressure Affected by Genetic Background?
Norio Takahashi, Munechika Misumi, Yasuharu Niwa, Hideko Murakami, Waka Ohishi, Toshiya Inaba, Akiko Nagamachi, Satoshi Tanaka, Ignacia Braga Tanaka, III, Gen Suzuki
Long-Term Effects of the Rain Exposure Shortly after the Atomic Bombings in Hiroshima and Nagasaki
Ritsu Sakata, Eric J. Grant, Kyoji Furukawa, Munechika Misumi, Harry Cullings, Kotaro Ozasa, Roy E. Shore
Exposure to Ionizing Radiation and Risk of Dementia: A Systematic Review and Meta-Analysis
Tanvi Srivastava, Ekaterina Chirikova, Sapriya Birk, Fanxiu Xiong, Tarek Benzouak, Jane Y. Liu, Paul J. Villeneuve, Lydia B. Zablotska