Hematological and biochemical parameters are useful indicators of raptor health. However, there is still a paucity of such data for many species, including the Black-chested Buzzard-Eagle (Geranoaetus melanoleucus), native to South America. The goal of this study was to determine hematology and blood chemistry reference intervals in 31 captive Black-chested Buzzard-Eagles held at a raptor rehabilitation center in central Chile. We report parameters of interest such as the mean ± SD, and reference intervals for this group of birds using 90% confidence intervals. Measured hematological parameters included red blood cell count, packed cell volume, hemoglobin concentration, Wintrobe indices, and total and differential leukocyte counts. Measured biochemical parameters included aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, and creatine kinase activities; and glucose, lipids, proteins, electrolytes, and uric acid plasmatic concentration. All Black-chested Buzzard-Eagle samples were negative for hemoparasites. Establishing reference intervals for hematological and biochemical parameters in the Black-chested Buzzard-Eagle adds to the body of knowledge of health indicators for this raptor species, contributes to its management and ex-situ conservation, and helps identify future research needs.