Cell therapies have shown promise for repairing the injured spinal cord in experimental models and are now being evaluated in clinical trials for the treatment of human spinal cord injury (SCI). To date, experimental evaluation of implanted cell survival, migration, and integration within the injured central nervous system (CNS) of animals has been technically demanding, requiring tissue sectioning, staining, imaging, and manual reconstruction of 2-dimensional (2D) specimens in 3 dimensions (3D). Not only are these histological procedures laborious and fraught with processing artifacts during manual 3D reconstruction, but they are time-intensive. Herein we describe the utility of 3D ultramicroscopy for assessment of cell therapies after SCI, a new state-of-the-art imaging modality in which whole brain and spinal cord samples are optically sectioned to allow evaluation of intact, macroscopic specimens with microscopic resolution.
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Original Article|
January 31 2012
The Utility of 3D Ultramicroscopy for Evaluating Cellular Therapies After Spinal Cord Injury
M. Ghosh;
M. Ghosh
1
The Miami Project to Cure Paralysis, University of Miami Miller School of Medicine, Miami, Florida, USA
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N. Jährling;
N. Jährling
4
Vienna University of Technology, Institute of Solid State Electronics, Department of Bioelectronics, Vienna, Austria
5
Center for Brain Research, Medical University of Vienna, Vienna, Austria
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M.C. Henao;
M.C. Henao
1
The Miami Project to Cure Paralysis, University of Miami Miller School of Medicine, Miami, Florida, USA
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H-U Dodt;
H-U Dodt
4
Vienna University of Technology, Institute of Solid State Electronics, Department of Bioelectronics, Vienna, Austria
5
Center for Brain Research, Medical University of Vienna, Vienna, Austria
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D.D. Pearse
D.D. Pearse
1
The Miami Project to Cure Paralysis, University of Miami Miller School of Medicine, Miami, Florida, USA
2
The Neuroscience Program, University of Miami Miller School of Medicine, Miami, Florida, USA
3
Department of Neurological Surgery, University of Miami Miller School of Medicine, Miami, Florida, USA
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Top Spinal Cord Inj Rehabil (2012) 18 (1): 73–76.
Citation
M. Ghosh, N. Jährling, M.C. Henao, H-U Dodt, D.D. Pearse; The Utility of 3D Ultramicroscopy for Evaluating Cellular Therapies After Spinal Cord Injury. Top Spinal Cord Inj Rehabil 1 January 2012; 18 (1): 73–76. doi: https://doi.org/10.1310/sci1801-73
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