Species-specific frequency of occurrence (“frequency”) across 41 historical collections from the Shoal Creek mainstem (“Mainstem frequency”) and 16 collections from four tributary streams (“Tributary frequency”) in the Shoal Creek system, for 31 fish species. Summed capture totals (“summed captures”) are the total numbers of individuals captured across 18 mainstem and 15 tributary collections for which the historical records include counts. All collection sites are in Dawson County, Georgia. Collection dates span 1948 to 2017 (mainstem) and 1950 to 2011 (tributaries). All data are archived and provided by the Georgia Museum of Natural History.