Improvements to the BNC images and dose-response calibration curves through the addition of hypotonic solution (75 mM KCl). Panel A: More spatially separated nuclei and MN, with more uniform DRAQ5 staining intensity was observed with the use of KCl. Panel B: The dose-response calibration curves representing the average of four donors showing the rate of MN/BNC using sample processing protocols with or without the use of KCl. Error bars represent the standard error of the mean.