Data acquisition template used to collect only single, highly-focused, DNA-positive cells. Panel A: Bivariate plot of BF aspect ratio versus BF area, allowing for the selection of single cells and the removal of doublets and small and large debris. Panel B: Bivariate plot of DRAQ5 gradient RMS versus BF gradient RMS enabling the selection of sharply-focused cells while eliminating blurry cells. Panel C: Histogram of DRAQ5 intensity allows for the selection of DNA positive cells and the elimination of very dimly stained cells.