Elimination of false positive artifacts. Panel A: Use of the cytoplasm mask to identify false-positive MN residing outside of the cytoplasm (top image) and the skeleton mask to identify artifacts residing between the two nuclei that are incorrectly identified as MN (middle image). Panel B: Use of the aspect ratio of the MN mask to identify elongated false-positive MN and the ratio of the median pixel intensity of the MN and BNC images to eliminate dim artifacts.