White blood cell (WBC) and lymphocyte (LYM) counts in the blood (panel A) and spleen (panel B) after irradiation and/or HLU. Mice were euthanized and blood was collected in EDTA-coated syringes via inferior vena cava at day 4 or 30 after unloading (day 11 and 37 postirradiation, respectively). Blood and spleens were analyzed using an automated hematology analyzer. Three-way ANOVA: *P < 0.05, main effect of proton radiation or HLU, ^†P < 0.05, irradiation × HLU interaction. ^TP < 0.1, irradiation × HLU interaction. Post hoc Tukey test: ^#P < 0.05, ^TTP < 0.1. Note the strong radiation dependence at day 4 after treatment, but smaller influence of HLU for both WBCs and lymphocytes.