A total of 471 samples of 292 wild boars (Sus scrofa) from Switzerland, Northern Italy, Germany, and Liechtenstein were collected during hunting. All samples (n=471, rectum and eye swabs, lung tissues samples) were screened for the presence of Chlamydiaceae by a 23S rRNA real-time quantitative PCR (qPCR). To determine the chlamydial species, positive samples (n=4) were tested by the Microarray assay and the 16S rRNA PCR, followed by sequencing. Three PCR methods detecting different tetracycline resistance gene determinants, such as tetR(C)-tetA(C), invasin-like (inv-like) region, and tetR(C), were applied to 204 samples from Italy and the canton of Ticino (Switzerland). A PCR detecting tetA(C) was performed on 407 samples from Italy and Switzerland (cantons AG, SO, TI, ZH). Positive samples (n=6) with the latter PCR were also investigated by the 16S rRNA PCR, followed by sequencing. IT=Italy; CH=Switzerland (including samples of Fürstentum Liechtenstein and Germany); TI=canton of Ticino; AG=canton of Aargau; SO=canton of Solothurn; ZH=canton of Zurich.