Molecular phylogenetic analysis showed the position of Hepatozoon canis isolates detected in red foxes (Vulpes vulpes) from southern France. The evolutionary history based on the Hepatozoon spp. 18S rRNA genes was inferred by using the maximum-likelihood method based on the Tamura-Nei model. The tree with the highest log-likelihood value (–1887.73) is shown. Initial tree(s) for the heuristic search were obtained automatically by applying neighbor-joining and BioNJ algorithms to a matrix of pairwise distances estimated using the maximum composite-likelihood approach and then selecting the topology with superior log-likelihood value. The analysis involved 48 nucleotide sequences. There were 518 positions in the final dataset.