Sliced specimen radiograph of a breast excision specimen serially sectioned into 18 slices (A through R). Circles demonstrate areas of calcification. Numbers represent blocks submitted. X marks blocks with ductal carcinoma in situ (DCIS). The extent of DCIS by the serial sequential sampling method measured 2.4 cm in the mediolateral plane (specimen ML, size 5.7 cm/18 slices = 0.3 cm average slice thickness; 0.3 cm × 8 slices = 2.4 cm). The size based on the calcification method is 2.1 cm (7 slices involved × 0.3 cm), by the blocks method is 3.9 cm (13 blocks × 0.3 cm), and the largest dimension measured on one slide was 1.7 cm