Precision irradiation establishes chronic hyposalivation in preclinical NSG-SGM3 model. Panel A: Kinetics of pilocarpine-stimulated saliva flowrate (µg/min) in mice treated with different precision- doses [19 males (n = 4–5 per group), 20 females (n = 5 per group) and 16 old females (n = 4 per group) were used]. Panel B: Sex- and age-specific significant differences in pilocarpine-stimulated saliva fraction in mice treated with different precision doses. Data in each sex group is calculated relative to sham-treated animals. Panel C: Precision-high-dose-irradiated mice displaying delayed onset of stimulated saliva flow in time (0, 2, 4, 6 months). Panel D: Barplot showing significant changes in saliva fraction at +3, +6 and +24 weeks in irradiated young male, female and old female mice relative sham-treated anim als.*P < 0.01; **P < 0.001 and ***P < 0.0001.