Necropsy reveals precision-dose-irradiation associated changes on salivary tissue, stroma, epithelial cell polarity, and integrity in preclinical model. Panel A: Baseline differences in wet weight of total salivary gland tissue (mg/g body weight) harvested at study endpoint (6 months postirradiation) from young male, female and old female mice exposed 0-7.5 Gy doses of radiation [19 male (n = 4–5 per group/radiation dose), 20 female (n = 5 per group/radiation dose) and 16 old female (n = 4 per group/radiation dose) were used]. ns = not significant; **P < 0.001. Panel B: Precision-dose-radiation-specific percent changes in the body weight adjusted wet weight of total salivary tissue in mg. Panel C: Illustration of precision-radiation-induced salivary tissue volume reduction compared to sham-treated young female mouse. Panel D: Representative photomicrograph of stained parotid, submandibular and sublingual glands of 3 young female NSG-SGM3 mice at 6 month postirradiation (0 and 7.5 Gy). Sections were stained with H&E (i), Sirius red (ii), trichrome (iii), and immunostained for EpCAM (iv), Aquaporin 5/beta-catenin (v) and NKCC1 (iv). Scale bars = 200µm.