Visualization of centrosomes by anti-CENP-C antibody in metaphase chromosome spread. Metaphase chromosome spreads prepared using NHDFs fixed in Carnoy's solution were stained with anti-CENP-C antibody (green fluorescence) and chromosomal DNA was stained with DAPI (red artificial color). Left panel; merged image, Middle panel; CENP-C signals, Right panel; DAPI signals. The scale bar represents 5 µm.