Figures 1–6. Figure 1 . Effect of arachidonic acid (AA) on [3H]-thymidine incorporation by MG63 cells cultured on titanium (Ti) surfaces of varying roughness. The MG63 cells were seeded onto plastic and Ti surfaces and cultured in Dulbecco's modified Eagle medium (DMEM) containing 10% fetal bovine serum (FBS) and antibiotics. At 80% confluence, the FBS concentration was decreased to 2% and the cultures continued to confluence. At confluence, the indicated concentrations of AA were added and the cultures continued for an additional 24 hours. At harvest, [3H]-thymidine incorporation was determined as described in the Materials and Methods. #P < .05, vs plastic; *P < .05, vs untreated control (no AA); ○P < .05, vs smooth Ti; ΔP < .05, vs rough Ti. Figure 2 : Effect of AA acid on alkaline phosphatase (ALP) specific activity of MG63 cells cultured on Ti surfaces of varying roughness. MG63 cells were seeded onto plastic and Ti surfaces and cultured to confluence in DMEM containing 10% FBS and antibiotics. At confluence, the indicated concentrations of AA were added and the cultures continued for an additional 24 hours. At harvest, ALP-specific activity was determined as described in the Materials and Methods. #P < .05, vs plastic; *P < .05, vs untreated control (no AA); ○P < .05, vs smooth Ti; ΔP < .05, vs rough Ti. Figure 3 : Effect of prostaglandin E2 on [3H]-thymidine incorporation by MG63 cells cultured on Ti surfaces of varying roughness. The MG63 cells were seeded onto plastic and Ti surfaces and cultured in DMEM containing 10% FBS and antibiotics. At 80% confluence, the FBS concentration was decreased to 2% and the cultures continued to confluence. At confluence, the indicated concentrations of prostaglandin E2 were added and the cultures continued for an additional 24 hours. At harvest, [3H]-thymidine incorporation was determined as described in the Materials and Methods. #P< .05, vs plastic; *P < .05, vs untreated control (no prostaglandin E2); ○P < .05, vs smooth Ti. Figure 4 : Effect of prostaglandin E2 on ALP- specific activity of MG63 cells cultured on Ti surfaces of varying roughness. The MG63 cells were seeded onto plastic and Ti surfaces and cultured to confluence in DMEM containing 10% FBS and antibiotics. At confluence, the indicated concentrations of prostaglandin E2 were added and the cultures continued for an additional 24 hours. At harvest, ALP-specific activity was determined as described in the Materials and Methods. #P < .05, vs plastic; *P < .05, vs untreated control (no prostaglandin E2); ○P < .05, vs smooth Ti. Figure 5 : Effect of indomethacin on [3H]-thymidine incorporation by MG63 cells cultured on Ti surfaces of varying roughness. The MG63 cells were seeded onto plastic and Ti surfaces and cultured in DMEM containing 10% FBS and antibiotics. At 80% confluence, the FBS concentration was decreased to 2% and the cultures continued to confluence. At confluence, indomethacin (100 nM) or vehicle was added and the cultures continued for an additional 24 hours. At harvest, [3H]-thymidine incorporation was determined as described in the Materials and Methods. #P < .05, vs plastic; ○P < .05, vs no indomethacin treatment; *P < .05, vs smooth Ti. Figure 6 : Effect of indomethacin on ALP-specific activity of MG63 cells cultured on Ti surfaces of varying roughness. The MG63 cells were seeded onto plastic and Ti surfaces and cultured to confluence in DMEM containing 10% FBS and antibiotics. At confluence, indomethacin (100 nM) or vehicle was added and the cultures continued for an additional 24 hours. At harvest, ALP-specific activity was determined as described in the Materials and Methods. #P < .05, vs plastic; ○P < .05, vs no indomethacin; *P < .05, vs smooth Ti; ΔP < .05, vs rough Ti