Figure 3.
(a) Oxidized surface in graft: Differences and limits between the basal bone (B) and the grafted region (R) were easily visible (asterisks). Bone structure was quite dense, but a high percentage of the mineralized tissues contained graft particles (Toluidine blue, basic fuchsin, original magnification ×8). (b) Oxidized surface in graft: Magnification of the regenerated tissues showed that many peri-implant trabeculae (B) contained embedded graft particles (G) and were quite thick and well connected. Most of the graft particles (G) were embedded in the newly formed bone and were not found in the marrow spaces (M) (Toluidine blue, basic fuchsin, original magnification ×25). (c) Oxidized surface in graft: Magnification of the previous case. The surfaces of the graft particles (G) exposed to the marrow spaces were covered by multinuclear giant cells (asterisk) and showed the typical white unstainable layer (B = bone, V = vessel, arrows = osteoclast) (Toluidine blue, original magnification ×200). (d) Oxidized surface in graft: The thickness of the titanium-oxide coating (arrows) well integrated to the newly formed bone (B) is clearly evident. The newly formed bone is composed of woven bone with small or large particles of the graft (G), with few medullary spaces containing vessels (V) (Toluidine blue, original magnification ×200). (e) Oxidized surface in graft: Small debris of the titanium-oxide coating was detected in macrophages a few microns from the implant surface without signs of acute or chronic inflammatory cell infiltrate. The black powder visible inside the macrophages represents small particles of the coating. The rectangle represents the area magnified in the following picture (NB = new bone, arrow = macrophages) (Toluidine blue, original magnification ×100). (f) Oxidized surface in graft: High-power magnification of the previous picture. Macrophages (M) engulfed by granules of TiUnite coating are visible in the peri-implant marrow spaces. (B = bone, O = osteocyte, arrows = bone-marrow interface, asterisk = mastcell) (Toluidine blue, original magnification ×1000)

(a) Oxidized surface in graft: Differences and limits between the basal bone (B) and the grafted region (R) were easily visible (asterisks). Bone structure was quite dense, but a high percentage of the mineralized tissues contained graft particles (Toluidine blue, basic fuchsin, original magnification ×8). (b) Oxidized surface in graft: Magnification of the regenerated tissues showed that many peri-implant trabeculae (B) contained embedded graft particles (G) and were quite thick and well connected. Most of the graft particles (G) were embedded in the newly formed bone and were not found in the marrow spaces (M) (Toluidine blue, basic fuchsin, original magnification ×25). (c) Oxidized surface in graft: Magnification of the previous case. The surfaces of the graft particles (G) exposed to the marrow spaces were covered by multinuclear giant cells (asterisk) and showed the typical white unstainable layer (B = bone, V = vessel, arrows = osteoclast) (Toluidine blue, original magnification ×200). (d) Oxidized surface in graft: The thickness of the titanium-oxide coating (arrows) well integrated to the newly formed bone (B) is clearly evident. The newly formed bone is composed of woven bone with small or large particles of the graft (G), with few medullary spaces containing vessels (V) (Toluidine blue, original magnification ×200). (e) Oxidized surface in graft: Small debris of the titanium-oxide coating was detected in macrophages a few microns from the implant surface without signs of acute or chronic inflammatory cell infiltrate. The black powder visible inside the macrophages represents small particles of the coating. The rectangle represents the area magnified in the following picture (NB = new bone, arrow = macrophages) (Toluidine blue, original magnification ×100). (f) Oxidized surface in graft: High-power magnification of the previous picture. Macrophages (M) engulfed by granules of TiUnite coating are visible in the peri-implant marrow spaces. (B = bone, O = osteocyte, arrows = bone-marrow interface, asterisk = mastcell) (Toluidine blue, original magnification ×1000)

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