This study reports on findings about the natural history of the Cloud-forest Pygmy-Owl (Glaucidium nubicola). Recently described as a species, the general ecology of the Cloud-forest Pygmy-Owl remains poorly documented. Three banded individuals at a private reserve in the province of Pichincha, Ecuador, are used to describe its habitat use, diet items, and individual longevity. Our observations suggest this local population prefers wet primary cloud forest and appears to have a generalist diet. Multiple recaptures of a banded individual indicate a longevity record of 5 years. Additionally, ectoparasites were found on 2 out of 3 captured individuals.