Migratory tracking of genetically distinct populations can be used to develop conservation strategies that prioritize the protection of unique genetic lineages across the annual cycle. In North America, the island of Newfoundland harbors populations of numerous species that are genetically differentiated from populations in mainland North America. The American Redstart (Setophaga ruticilla) is a widespread Neotropical migratory songbird that breeds across North America and has a mitochondrial haplogroup unique to the Newfoundland breeding population. Stable-hydrogen isotope analyses have broadly identified the Caribbean islands as the nonbreeding locations for American Redstarts breeding in northeastern North America, but the specific nonbreeding sites for the Newfoundland breeding population remain unclear. The objective of this study was to use mitochondrial haplogroups to elucidate nonbreeding locations of the Newfoundland population of the American Redstart. We sampled 180 individuals from 9 locations across the Caribbean and sequenced the mitochondrial control region. We identified 4 individuals with the Newfoundland haplogroup in Puerto Rico (n = 3) and the Dominican Republic (n = 1). However, we primarily detected individuals with other haplogroups at these 2 nonbreeding sites. Our results suggest that the Newfoundland breeding population of the American Redstart has a restricted nonbreeding range (Puerto Rico and Dominican Republic) and mixes with individuals from other breeding populations at these sites. These findings contribute to a better understanding of how American Redstart populations are connected across the annual cycle, improving our understanding of population ecology and evolution.

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